Bio-One Of Orange decontamination and biohazard cleaning services

Learning About the Four Biosafety Levels

Learning About the Four Biosafety Levels - Bio-One of Orange.

It takes special training and preparation to remove biohazardous waste. Work environments involving biological waste, such as laboratories, medical facilities, and chemical labs, follow strict guidelines regarding adequate PPE and practices to ensure that every material handled inside these areas doesn’t spread outside the parameters.

What is Biosafety? 

Every safety precaution that is applied in work environments to reduce employees' exposure to potentially infectious microorganisms and bacteria is considered biosafety. Bacteria and airborne pathogens can easily result in disease outbreaks if agencies, facilities, and employers fail to follow these regulations.

Bio-One of Orange works with law enforcement and local authorities to respond effectively when these need to remove biohazardous waste from premises affected by crime and trauma scenes. Our ultimate goal is to protect the community from these dangerous pathogens. 

4-Level Biosafety Classification - How does it work?

There are four levels of biosafety practices. Each level builds upon the other, starting from Biosafety Level 1 (BSL-1), representing the “lowest” risk of disease, infection, and potential biohazard. Laboratories and work environments classified in a Level 4 (BSL-4) are those that handle the most dangerous and exotic bacteria/pathogens; the outbreak of these materials can be fatal and mostly impossible to treat with medication or vaccination.

Biosafety Level 1(BSL-1) 

Microbes with the lowest or unknown risk of consistently causing diseases in healthy adults. Workplaces designated with BLS-1 classification usually present minimal risk to lab facilities and environments.

Even so, workers must adhere to the following regulations and safety practices:

  • Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. 
  • A sink must be available for hand washing/disinfection.
  • The lab area should have doors that separate this workspace from the rest of the facility.

Facilities designated with BSL-1 classification include Research labs with the use of benches.

Biosafety Level 2 (BSL-2)

Built upon BSL-1, environments designated with a BLS-2 classification work with microbes that pose a moderate risk of exposure. Microbes are typically indigenous (microorganisms that have been adapting and surviving within the native soil environments throughout the years), and the intensity of the associated diseases may variate.

Regulations and safety practices include:

  • Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. Emphasis on eye protection and face shields, if necessary.
  • A sink and eyewash must be available for disinfection.
  • Use of autoclave or specific alternative methods to dispose of biological waste.

Facilities designated with BSL-2 classification include Laboratories with self-closing doors. Entrance is restricted.

Biosafety Level 3 (BSL-3) 

Built upon BLS-2, environments designated with a BLS-3 classification work with microbes that may carry potentially lethal diseases via respiratory transmission. Microbes can be either indigenous or exotic

Regulations and safety practices include:

  • Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. Respirators may be required. 
  • All microbe work must be performed using a Biological Safety Cabinet (BSC).
  • A hands-free sink and eyewash must be available for disinfection.
  • The laboratory has an airflow system that prevents the exhausted air from coming into clean areas.

Facilities designated with BSL-3 classification include Laboratories with two sets of self-closing and locking doors. Access to the laboratory is restricted/controlled at all times.

Biosafety Level 4 (BSL-4) 

Built upon BLS-3, environments designated with a BSL-4 classification work with the most dangerous and exotic bacteria and microbes. Diseases and infections caused by these microbes can be aerosol-transmitted and are usually fatal.

Regulations and safety practices include:

  • Mandatory change of clothing before and after exiting the laboratory. A Post-exit shower is also mandatory.
  • Decontamination of all used materials before exiting the laboratory.
  • All microbe work must be performed using a Class III Biological Safety Cabinet (BSC).
  • Adequate Personal Protective Equipment (PPE) - Full bodysuit with air supply and positive pressure system.

Facilities designated with BSL-4 classification include Laboratories in a separate building or isolated from the rest of the building. Laboratories must have dedicated air supply and exhaust air systems and their own decontamination systems.

Bio-One or Orange can help.

Bio-One of Orange's certified technicians follow strict guidelines and procedures to remove biohazardous waste from contaminated scenes. Whether from blood and bodily fluids, medical waste, gases, or other biological agents, our specialists are trained to contain and dispose of the waste effectively and timely properly.

Bio-One of Orange can safely remove biohazardous waste from any area.
Bio-One of Orange can safely remove biohazardous waste from any area.

Help First, Business Second!

Bio-One of Orange offers a quick solution to life’s most difficult and unexpected situations. Locally owned, we provide top-quality, industry-leading cleaning, sanitation, and decontamination services in:

Recovery Services

  • Blood spill and biohazard cleanup services
  • Crime and trauma scene cleanup services
  • Bodily fluids cleanup services
  • Feces and urine cleanup services
  • Undiscovered death cleanup services
  • Homicide/Suicide cleanup services
  • Virus Disinfection
  • Medical waste disposal services
  • Fentanyl cleanup services
  • Mold remediation services
  • Odor removal services
  • Sewage backup cleanup services

Hoarding Cleanup & Clutter Removal Services

  • Hoarding cleanup services
  • Animal hoarding cleanup services
  • Rodent droppings removal services
  • Gross filth cleanup services
  • Declutter and junk removal
  • Deep clean
  • Hazardous waste removal


Locally owned, Bio-One of Orange is proud to serve Orange County and surrounding Southern California areas: Aliso Viejo, Anaheim, Brea, Buena Park, Costa Mesa, Cypress, Dana Point, Fountain Valley, Fullerton, Garden Grove, Huntington Beach, Irvine, La Habra, La Palma, Laguna Beach, Laguna Hills, Laguna Niguel, Laguna Woods, Lake Forest, Los Alamitos, Mission Viejo, Newport Beach, Orange, Placentia, Rancho Santa Margarita, San Clemente, San Juan Capistrano, Santa Ana, Seal Beach, Stanton, Tustin, Villa Park, Westminster, and Yorba Linda!

Our professional technicians are ready to help you restore your home or business. Bio-One of Orange, a proud member of the Orange Chamber of Commerce, is available 24/7, 365 days a year. Contact us at 714-397-8375.